Vladkova T.G.Martinov B.L.Staneva A.D.Ivanova I.A.Gospodinova D.N.Albu-Kaya M.G.2024-07-162024-07-162024-07-162024-07-162023-01-011314-79781314-7471SCOPUS_ID:85163783941https://rlib.uctm.edu/handle/123456789/1433The aim of this investigation was to develop collagen based composite biomaterial with improved antimicrobial activity using a combination of antimicrobial agents consisting of zinc titanate embedded in a silane matrix, (ZnTiO3/ SiO2) and fucoidan at varied concentrations. The morphology of the investigated porous collagen/(ZnTiO3/SiO2)/ fucoidan composites was observed by SEM and their antimicrobial activity was evaluated against four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida, Salmonela holeresius), two Grampositive bacteria (Staphylococcus epidermidis, Bacillus cereus) and two fungi (Candida Lusitania, Saccharomyces cerevisiae) by disk diffusion test. Broad-spectrum antimicrobial activity of the studied porous collagen/(ZnTiO3/SiO2)/fucoidan composites was demonstrated, specific toward the different test microbial strains and dependent on the fucoidan concentration. The specific activity toward different microbial cultures was ascribed to the features of the microbial cells (size, shape, cell wall and membrane) and differences in the composition of the secreted exopolymeric substances. It was found that both, the formed interconnected open porous structure of the mixed collagen/fucoidan matrix with fine dispersed submicron ZnTiO3/SiO2 particles along the marix fibrils and the own antibacterial activity of the fucoidan, contribute to the increased wide spectrum antibacterial activity compared to that of similar collagen composites do not containing fucoidan.enArticle