Pechlivanova D.M.Assenov B.V.Mateeva P.I.Dzhambazova E.B.Peneva P.N.Todorov P.T.2024-07-162024-07-162024-07-162024-07-162023-01-011314-79781314-7471SCOPUS_ID:85152139368https://rlib.uctm.edu/handle/123456789/1426Endogenous peptides have various modulating effects on many physiological and pathological processes, including the processing of pain information. Hemorphins are hemoglobin-derived peptides with affinity for both opioid receptors and insulin-regulated aminopeptidase. We recently reported on the antinociceptive effects of hemophins of different lengths and amino acid composition after their supraspinal injection. The present study aims to elucidate the potential effects of locally (intraplantar) injected hemorphin VV-H7 (Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe-NH2) and its analogs with one or two Arg-Gly-Asp sequence on carrageenaninduced hyperalgesia in rats. We used indomethacin as a reference anti-inflammatory analgesic. Pain thresholds and paw volume were measured before and at 1, 3, and 4 hours after intraplantar carrageenan injection using a paw pressure test and plethysmometry in male Wistar rats. The precursor peptide VV-H7 showed an antihyperalgesic effect comparable to that of indomethacin, while its peptide analogues one or two Arg-Gly-Asp sequence showed an acceleration of the antinociceptive effect, but shorter duration of antihyperalgesia and lack of efficacy against edema.enArticle