Suleymanoglu Y.Borisova B.Danalev D.Petrov A.Tafradjiyska R.Nocheva H.2026-01-202026-01-202026-01-202026-01-202025-01-010428-029610.3897/pharmacia.72.e152026SCOPUS_ID:105016689240https://rlib.uctm.edu/handle/123456789/1892Adaptation to elevated temperatures poses a challenge that involves large-scale metabolic changes. It also affects the pharmacokinetics and pharmacodynamics of exogenous substances. Our experiments clearly showed differences in paw pressure thresholds in rats intraperitoneally injected with TDIFELLK-a C-terminal fragment of the calcium-binding, spermatid-specific protein 1- before and after exposure to 38.5 ± 0.5°C, compared to administration at 22 ± 2°C. Similarly, the analgesic effect of the substance, following antagonism of key analgesic receptors-namely opioid, cannabinoid, and serotonergic-was differentially influenced by heat exposure at normal temperature. The administration of the substance before heat exposure exerted a stronger effect than administration after heat exposure.enArticle