Lazarević J.Zvezdanović J.Anastassova N.Mavrova A.T.Yancheva D.Šmelcerović A.2024-07-102024-07-102024-07-102024-07-102022-01-012217-25210351-608310.5937/afmnai39-36399SCOPUS_ID:85148094933https://rlib.uctm.edu/handle/123456789/785Introduction: Oxidative stress and resulting lipid peroxidation are involved in numerous pathological conditions. For this reason, the role of antioxidants attracts attention and the radical-scavenging capacity of many natural and synthetic supplements and drugs has been extensively evaluated. Material and methods: In the present study, seven N,N'-disubstituted benzimidazole-2-thiones with ester (1 - 4) and hydrazide (5 - 7) side chains were investigated for in vitro antioxidant activity using lipid peroxidation method. Results: Among the assayed compounds, three hydrazides, 1,3-bis[3-(hydrazinooxy)-3-oxopropyl]-1,3- dihydro-2H-benzimidazole-2-thione (5), 1,3-bis[3-(hydrazinooxy)-3-oxopropyl]-5-methyl-1,3-dihydro-2Hbenzimidazole- 2-thione (6) and 1,3-bis[3-(hydrazinooxy)-3-oxopropyl]-5-benzoyl-1,3-dihydro-2Hbenzimidazole- 2-thione (7) showed good antioxidant properties (IC50 < 100 μM), with the best lipid peroxidation inhibition values (IC50) shown for compound 5 (64 ± 10 μM) and compound 6 (73 ± 29 μM). Conclusion: Indicated hydrazide structures may constitute a sort of molecular basis, a promising starting point for the development of compounds for the prevention and treatment of diseases resulting from oxidative damage.enArticle