Ivanov T.Petrin S.Lalov I.2024-07-162024-07-162024-07-162024-07-162023-01-011314-79781314-7471SCOPUS_ID:85164608753https://rlib.uctm.edu/handle/123456789/1435In current paper a comparative study of bioethanol yields from ryegrass biomass by different type of pretreatment and enzyme hydrolysis is done. Acidic and alkali hydrolysis was used as a pretreatment of this feedstock. Cellulase blend 10 FPU g-1 dry matter was used for enzyme hydrolysis. The pre-treatment was conducted by autoclaving for 20 minutes at 121°C. Best results were obtained by pretreatment with 1 % HCl and 1 % NaOH. Hydrolysate obtained by pretreatment with hydrochloric acid contained 33.6 mg mL-1 reducing sugars, and that obtained by pretreatment with base - 29.0 mg mL-1. Both hydrolysates were found to be suitable substrates for bioethanol generation, where 5.55 g ethanol/100 g dry matter was produced from grass hydrolysate obtained by acidic hydrolysis followed by enzyme hydrolysis and from alkali pretreated hydrolysate-4.73 g ethanol/100 g dry matter. The results demonstrated that ryegrass biomass is suitable feedstock for bioethanol production.enArticle