University of Chemical Technology and Metallurgy

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Browsing University of Chemical Technology and Metallurgy by Author "Abarova S."

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    Spectroscopic and thermodynamic characterization of the interaction of a new synthesized antitumor drug candidate 2H4MBBH with human serum albumin
    (2024-01-01) Abarova S.; Stoitchkova K.; Tzonev S.; Argirova M.; Yancheva D.; Anastassova N.; Tenchov B.
    In the present work we studied the interactions of a newly synthesized drug candidate, 2-(2-hydroxy-4-methoxyben-zylidene)-1-(1H-benzimidazol-2-yl)hydrazine (2H4MBBH), with human serum albumin (HSA) by fluorescence spectroscopy. 2H4MBBH-HSA binding parameters were assessed by fluorescence quenching strategy. As made clear by the concentration data, 2H4MBBH unequivocally quenched the instrinsic HSA fluorescence. The calculated Stern-Volmer quenching constant Ksv, the Ka of 2H4MBBH-HSA complexes, as well as the thermodynamic parameters ΔH°, ΔS° and ΔG°, showed that the H-bonding forces play major part in the interaction of 2H4MBBH with HSA. These calculations point to a quenching component based on 2H4MBBH-HSA static complex formation rather than energetic collisions.