Browsing by Author "Amara D.G."

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    Lobularia libyca: Phytochemical Profiling, Antioxidant and Antimicrobial Activity Using In Vitro and In Silico Studies
    (2022-08-01) Benchikha N.; Chelalba I.; Debbeche H.; Messaoudi M.; Begaa S.; Larkem I.; Amara D.G.; Rebiai A.; Simal-Gandara J.; Sawicka B.; Atanassova M.; Youssef F.S.
    Lobularia libyca (L. libyca) is a traditional plant that is popular for its richness in phenolic compounds and flavonoids. The aim of this study was to comprehensively investigate the phytochemical profile by liquid chromatography, electrospray ionization and tandem mass spectrometry (LC-ESI-MS), the mineral contents and the biological properties of L. libyca methanol extract. L. libyca contains significant amounts of phenolic compounds and flavonoids. Thirteen compounds classified as flavonoids were identified. L. libyca is rich in nutrients such as Na, Fe and Ca. Moreover, the methanol extract of L. libyca showed significant antioxidant activity without cytotoxic activity on HCT116 cells (human colon cancer cell line) and HepG2 cells (human hepatoma), showing an inhibition zone of 13 mm in diameter. In silico studies showed that decanoic acid ethyl ester exhibited the best fit in β-lactamase and DNA gyrase active sites; meanwhile, oleic acid showed the best fit in reductase binding sites. Thus, it can be concluded that L. libyca can serve as a beneficial nutraceutical agent, owing to its significant antioxidant and antibacterial potential and due to its richness in iron, calcium and potassium, which are essential for maintaining a healthy lifestyle.
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    Nutritional potential, phytochemical analysis, and biological activities of quinoa (Chenopodium quinoa Willd.) seeds from arid zone culture
    (2024-01-01) Touil L.; Rami R.; Aydi S.S.; Amara D.G.; Messaoudi M.; Sawicka B.; Atanassova M.; Zahnit W.; Aydi S.; Ahmad S.F.; Mars M.
    Quinoa, scientifically known as Chenopodium quinoa Willd., is well recognized for its exceptional nutritional composition and potential benefits for human health. This research aims to conduct a phytochemical analysis of nutraceutical properties and biologcal activities of seven different quinoa cultivars, namely, red, white, Amarilla marangani, kancolla, Giza 02, Amarilla sacaca, and black. The analysis of ground seeds revealed that Giza 02, white, and A. marangani had the most significant protein, fat, ash, and carbohydrates, respectively. Furthermore, the MeOH extracts of the various quinoa genotypes exhibited high levels of total phenolic, flavonoid, condensed tannin and reducing sugar contents in the seed extracts obtained using consecutive solvents. In addition, HPLCDAD analysis revealed the presence of seven phenolic compounds, which may be classified into four families: phenolic acid, flavonoids, coumarins, and stilbenes. The IC50 values for the antioxidant activity ranged from 6.4 to 7.7 μg/mL. Furthermore, the variability in the antibacterial activity across different genotypes and gram-positive and gram-negative bacterial species was observed, and the efficacy of the CYHA-red extract against Micrococcus luteus was the highest. The findings of this study indicate that the incorporation of quinoa into the arid and Saharan cultivation system has the potential to enhance the nutritional and nutraceutical characteristics of the seeds.