Browsing by Author "Georgiev D."

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    Study on the synthesis, characterization and bioactivities of 3-methyl-9'-fluorenespiro-5-hydantoin
    (2016-01-01) Marinova P.; Marinov M.; Kazakova M.; Feodorova Y.; Slavchev A.; Blazheva D.; Georgiev D.; Penchev P.; Sarafian V.; Stoyanov N.
    This work describes a method for synthesis, as well as in vitro antiproliferative and antibacterial investigation of 3-methyl-9'-fluorenespiro-5-hydantoin. The structure of the substituted fluorenylspirohydantoin derivative was verified by UV-Vis, FT-IR, Raman, 1H NMR and 13C NMR spectroscopy, and by using a combination of 2D NMR experiments, which included 1H-1H COSY, HMQC and HMBC sequences. The geometry of the compound was optimized by the B3LYP density functional with 6-31G(d) basis set and the 1H and 13C NMR spectra were predicted with the HF/6-31G(d) calculations at the optimized geometry. The anticancer activity of the 3-methyl-9'-fluorenespiro-5-hydantoin was determined in suspension cell lines originating from tumors in humans (WERI-Rb-1). The cytotoxic effect was evaluated by WST-assay (Roche Applied Science). The antimicrobial effect of the compound against Gram-negative, Gram-positive bacteria and the yeast Candida albicans was investigated.
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    Synthesis and antimicrobial study of new Pt(IV) and Ru(III) complexes of fluorenylspirohydantoins
    (2019-01-01) Marinova P.; Marinov M.; Georgiev D.; Becheva M.; Penchev P.; Stoyanov N.
    The present work presents a synthesis, characterization and antimicrobial activitiy of new Pt(IV) and Ru(III) complexes of (9’-fluorene)-spiro-5-hydantoin (HL1) and (9’-fluorene)-spiro-5-(2-thiohydantoin) (HL2). The complexes were investigated by elemental analysis, UV-Vis and IR spectroscopy. The free ligands were studied by UV-Vis, IR, 1H NMR, 13C NMR and Raman spectroscopy. The antimicrobial tests of HL1 showed week bacteriostatic effect against Staphylococcus aureus ATCC 6538. The results for [Pt(L1)2(H2O)2(OH-)2] complex showed a good antimicrobial activity against Esсherichia coli ATCC 8739, Bacillus licheniformis ATCC 6633, S. aureus and the absence of such effect on the yeasts. The HL2 and its [Pt(L2)2(H2O)2(OH-)2] and [Ru(L2)3(H2O)3] complexes do not possess antimicrobial activity against the tested bacteria and yeasts.