Browsing by Author "Georgieva S."
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Item Assessment of Physical-Chemical Characteristics of Surface Water from Key Sites of the Mesta River: State and Environmental Implications(2018-06-12) Georgieva S.; Gartsiyanova K.; Ivanova V.; Vladimirova L.The anthropogenic source pollution of the Mesta River was assessed during the period 2011 and 2016 in terms of pH, conductivity, chemical oxygen demand (COD), anions and heavy metals in key sites of the Mesta river pointed as: S1 (the Mesta river before the Iztok river); S2 (the Iztok river before the Mesta river); S3 - the river Mesta after the Iztok river); S4 - the river Mesta at Momina Klisura, near Bukovo) and S5 - the river Mesta before the Greece border, after the Matnitsa river. The application of multivariate clustur analysis (CA) for the interpretation of a large and complex data matrix obtained during a monitoring program of surface water in Mesta river is presented in this study. The dataset consists of analytical results from a 6-yrs survey conducted in selected points of the river system. The physical-chemical characterization in the water samples were made in accordance with the Directive 2000/60/EU-Water Framework Directive (WFD) and its equivalent criteria transposed into the Water Law (WL) in Bulgaria. In water, concentrations (mg L-1) during 2016 of NO2 - (0.006 to 0.052), NO3 -(0.01 to 1.33) and total contents in μg L-1 of Cu (<0.002), Pb (<0.003), Co(<0.002), Ni(<0.003), S(<0.050) and Zn(<0.02), pH (5.60 to 8.00), and electrical conductivity (0.12 to 48.60 mS.cm-1) were agreed with environmental standards except cadmium Cd (>0.15 μg L-1) and PO4 3-(0.15 mg L-1). During analyzed period the cadmium concentration was much higher than recommended limit only. In this sense, it was possible to demonstrate relatively good quality of river water even with numbers of industrial and touristic activities in the analyzed area but also to consider a future concept on cadmium sources and their eliminated.Item Chemical Behavior and Bioactive Properties of Spinorphin Conjugated to 5,5′-Dimethyl- and 5,5′-Diphenylhydantoin Analogs(2024-06-01) Georgieva S.; Todorov P.; Tchekalarova J.; Subaer S.; Peneva P.; Chakarov K.; Hartati H.; Faika S.The discovery of new peptides and their derivatives is an outcome of ongoing efforts to identify a peptide with significant biological activity for effective usage as a possible therapeutic agent. Spinorphin peptides have been documented to exhibit numerous applications and features. In this study, biologically active peptide derivatives based on novel peptide analogues of spinorphin conjugated with 5,5′-dimethyl (Dm) and 5,5′-diphenyl (Ph) hydantoin derivatives have been successfully synthesized and characterized. Scanning electron microscopy (SEM) and spectral methods such as UV-Vis, FT-IR (Fourier Transform Infrared Spectroscopy), CD (Circular Dichroism), and fluorimetry were used to characterize the microstructure of the resulting compounds. The results revealed changes in peptide morphology as a result of the restructuring of the aminoacidic sequences and aromatic bonds, which is related to the formation of intermolecular hydrogen bonds between tyrosyl groups and the hydantoin moiety. Electrochemical and fluorescence approaches were used to determine some physicochemical parameters related to the biological behavior of the compounds. The biological properties of the spinorphin derivatives were evaluated in vivo for anticonvulsant activity against the psychomotor seizures at different doses of the studied peptides. Both spinorphin analog peptides with Ph and Dm groups showed activity against all three phases of the seizure in the intravenous Pentylenetetrazole Seizure (ivPTZ) test. This suggests that hydantoin residues do not play a crucial role in the structure of spinorphin compounds and in determining the potency to raise the seizure threshold. On the other hand, analogs with a phenytoin residue are active against the drug-resistant epilepsy test (6-Hz test). In addition, bioactivity analyses revealed that the new peptide analogues have the potential to be used as antimicrobial and antioxidant compounds. These findings suggest promising avenues for further research that may lead to the development of alternative medicines or applications in various fields beyond epilepsy treatment.Item Comparison of citrus peels as a source of valuable components - Polyphenols and antioxidants(2013-11-05) Karsheva M.; Kirova E.; Alexandrova S.; Georgieva S.The aim of this study is to compare some citrus fruits' peels: mandarins and grapefruits, as sources of polyphenolic compounds, to determine the antioxidant activity of the extracts and to find the effect of the operational conditions on the final extract properties. It was found that the both materials are rich of polyphenols and exhibit high antioxidant activity; better results are obtained for the grapefruit peels. The flavonoids' content in both materials studied is quite close. The solid-to-liquid ratio plays an important role in the extraction process. The better results are obtained for the ratio of 1:10. The results of this study confirm that the citrus peels are promising source of natural antioxidants and polyphenols.Item Complex activity and sensor potential toward metal ions in environmental water samples of n-phthalimide azo-azomethine dyes(2021-10-01) Georgieva S.; Bezfamilnyi A.; Georgiev A.; Varbanov M.Herein, the spectral and electrochemical characterizations of three different substituted N-phthalimide azo-azomethine (NAA) dyes (L) containing an o-hydroxy group and their NAA-M(II) chelates [M(II): Cu, Ni, Co, Pb] were reported by using UV–Vis and fluorescence spectroscopy and potentiometric and voltamperometric techniques. The pK value of the dyes as well as the stoichiometry and stability of the NAA-metal chelates were studied, and the stoichiometry was found to be mostly 1:2 (ML2 ) with high complex stability constant values. The sensor activity of N-phthalimide azo-azomethine derivatives toward pH and metal ions has been also investigated and tested for indicator application in acid–base analysis and detection of Cu(II) ions in real samples of surface river water using voltamperometric detection. The results showed that one of the ligands possesses the highest electrochemical response upon binding to copper ions and could be successfully used in the analysis of copper in water at a concentration range of the analyte from 3.7 × 10−7 to 5.0 × 10−6 mol L−1, with analytical characteristics of the method being Sr = 1.5%, LOD = 3.58 µg L−1 and LOQ =11.9 µg L−1.Item DEVELOPMENT OF VOLTAMMETRIC METHOD FOR DETERMINATION OF TRACE OF Cu(II) IN THE PRESENCE OF Fe(III) IN WATER USING 5,5'-DIPHENYLIMIDAZOLIDINE-2,4-DIONE DERIVATIVE(2021-01-01) Georgieva S.; Todorov P.; Bezfamilnyi A.A new cyclic voltammetric method has been developed for the determination of Cu(II) in the presence of Fe(III) in tap and surface water samples using complexing properties of 5,5'-diphenylimidazolidine-2,4-dione derivative. In acetate buffer solution (0.1 mol L-1; pH 5.25 ± 0.01) containing minimum ten-fold excess of imidazolidine derivative, copper metal ion was determined as complex compound following redox process into the working hanging mercury drop electrode (HMDE) versus Ag/AgCl, 3 mol L-1 KCl using as a reference electrode. Limits of detection as low as 0.0415 mg L-1 Cu(II) was achieved. The interfering effect of various cations as K(I), Na(I), Mg(II), Ca(II), Al(III), Zn(II), Fe(II), Fe(III) and anions: Cl-, NO3-, SO42-, PO43-, most common in surface and tap water samples, were also assessed.Item Influence of the alkaline electrolyte bath on the oxygen content of REBa2Cu3OY (RE = Y, Gd) superconducting ceramics(2013-12-17) Georgieva S.; Stoyanova-Ivanova A.; Nedeltcheva T.The oxygen stability of the REBa2Cu3Oy (RE = Y, Gd; y = 6,5+δ) superconducting ceramics previously immersed in strong alkaline electrolyte bath have been studied. The samples were placed in solution of 7M KOH (pH 13) for 96 h and then the oxygen content was determined by spectrophotometry and XRD-spectrometry. The results obtained by the both methods are identical in the limit of random errors. Consequently the electrolyte treatment of the samples does not influence the oxygen content. The critical temperature and the structure of the ceramics do not change also and these superconducting ceramics can be used in the electrotechnical devices.Item Investigation of electrochemical behavior of new hemorphin-5 analogue(2019-01-01) Georgieva S.; Todorov P.; Peneva P.A biologically active VV-hemorphin-5 (Valorphin: Val-Val-Tyr-Pro-Trp-Thr-Gln) analogue was characterized electrochemically. In this regard the oxidation and adsorption reactions of the peptide on an electrode surface in a phosphate buffer solution have been studied and compared with those of valorphin and polarographic active tryptophan and tyrosin. For investigation of the interfacial behavior of the VV-hemorphin-5 derivative (H2) voltamperometry with platinum electrode as working electrode, with Ag/AgCl, (3 mol L-1) KCl electrode as a reference electrode, and a carbon electrode as an auxiliary electrode, have been used. The redox potentials were determined under various mode of sweep - differential pulse (DP) and cyclic voltamperometry (CV). Analysis of the wave shape, pH dependence, and concentration dependence of the oxidation process leads to the conclusion that the valorphin derivative is adsorbed on the Pt-electrode. The detection of a low concentration (2x10-5 mol L-1) leads to the conclusion for possible application of the voltamperometry as a method for determination of low concentrations from this class peptides.Item Metal–Peptide Complexes with Antimicrobial Potential for Cotton Fiber Protection(2023-02-01) Georgieva S.; Todorov P.; Staneva D.; Grozdanov P.; Nikolova I.; Grabchev I.A study of the formation of copper (II) complexes with hemorphin peptide motifs in alkalic water solutions is presented. The effect of the peptide ligand on the complexing properties of the Cu (II) ion was quantified by giving the stoichiometry and stability of the complex compounds in the medium in which they are formed using voltammetric (cyclic) and spectral (UV-Vis and fluorimetric) analytical techniques. The resulting complexes were examined via IR spectroscopy to detect M-N and M-O oscillations and using the EPR approach in solution and in the solid phase to view the coordination and ligand binding regime. The possibility of the synergistic action of copper ions in the antivirus protection processes of cotton fibers coated in the same solvent with the newly obtained complex compounds was also investigated. One of the advantages is the formation of the complexes in an environment where the immobilization takes place, which contributes to increasing the efficiency of the process. The obtained results may serve as an aid for future more detailed biological studies of structure–activity relationships (SARs).Item OPTICAL PROPERTIES OF PAZO POLYMER COMPOSITE FILMS DOPED WITH PARTICLES OF A NOVEL COPPER HYDANTOIN COMPLEX(2021-01-01) Lilova V.; Trifonova Y.; Stoilova A.; Georgieva S.; Todorov P.In this work the transmittance coefficient (T), the reflectance coefficient (R), the refractive index (n), the extinction coefficient (k), the optical absorption coefficient (a) and the optical band gap (Eof thin (poly[1-[4-(3-carboxy-4-hydroxyphenylazo) benzenesulfonamido]-1,2-ethanediyl, sodium salt]), shortly PAZO, azopolymer films with embedded particles of Cu(II) 3-amino-5,5‘-dimethylhydantoin at three different concentrations have been determined. The influence of the dopants concentration on the optical performance of the composite films has been discussed.Item Photoinduced birefringence in pazo polymer nanocomposite films with embedded particles of biologically active metal complexes(2019-01-01) Mateev G.; Stoilova A.; Nazarova D.; Nedelchev L.; Todorov P.; Georgieva S.; Trifonova Y.; Lilova V.The composite materials based on azo polymers doped with metal particles attract considerable attention due to their advantageous electrical and optical properties. The present communication reports results from measuring the photoinduced birefringence in composite films of the azopolymer (poly[1-[4-(3-carboxy-4-hydroxyphenylazo) benzenesulfonamido]-1,2-ethanediyl, sodium salt]), shortly PAZO, doped with metal complexes of Cu(II) 3-amino-5,5'-dimethylhydantoin (CLP) and Ni(II) 3-amino-5,5'-dimethylhydantoin (NLP) of different concentrations. The thin film materials are prepared via spin coating. The thickness of the samples is in the range between 500 nm and 800 nm. The photoinduced birefringence is measured by a classical polarimetric setup, where a vertically polarized He-Cd laser emitting at ? = 442 nm is used as a pump laser, while a DPSS laser emitting at = 635 nm is used as a probe laser. The influence of the dopants concentration on the maximal induced birefringence and the time of response are discussed.Item Recent Synthesis, Characterization, and Pharmacological Evaluation of Multifunctional Hemorphins Containing Non-Natural Amino Acids with Potential Biological Importance(2022-11-01) Todorov P.; Georgieva S.; Tchekalarova J.The endogenous hemorphins are bioactive peptides with activity on opioid receptors. They are extensively studied and summarized in numerous reviews. During the last decade, several research teams have synthesized, characterized, and pharmacologically evaluated synthetic hemorphin analogs containing unusual amino acids, D-amino acids, α-aminophosphonic acids, and their derivatives. The present review summarizes the current studies on short-chain synthetic hemorphin peptide derivates containing non-natural amino acids. This review focuses on the structure–activity relationship analysis, details on specific methods for their characterization, and the advantage of synthetic hemorphin analogs compared to endogenous peptides as potent biologically active compounds with a complex mechanism of action.Item Speciation Analysis of Arsenic in Copper Electrolyte Baths Using Liquid-liquid Extraction and ICP-OES Method Detection(2022-01-01) Stefanov E.; Georgieva S.The present study describes a method for the determination of As (III) and As (V) in copper electrolytes. The method is based on the separation of As (III ) from a copper electrolyte by triple liquid-liquid extraction using a non-polar organic solvent in a medium of 10-12 mol L-1HCl. The extract contains As (III) and the raffinate-As (V), respectively. As(III) specie can be re-extracted from the organic solvent through the water. Analyzes of the concentration of As in the re-extract and raffinate were performed by ICP-OES spectroscopic method. The average recovery of arsenic by the proposed method is about 99%. Repeatability was estimated with RSD (n = 6). Selectivity and accuracy were proven by the standard addition method. The relative error for restoring the standard addition of As (III) is about 0.3%. The speciation method analysis could be applied for determination of the arsenic species in the analytical quality control of refined copper in copper tanks in the production of copper cathodes.Item Spectrophotometric method for rapid estimation of the oxygen stoichiometry in YBCO superconducting samples(2013-03-01) Georgieva S.; Nedeltcheva T.; Vladimirova L.; Stoyanova-Ivanova A.Detailed investigations on the possibility for determination of the oxygen stoichiometry in YBa2Cu3Oy superconducting samples by measuring the absorbance of the colored Co(III)-EDTA and Cu(II)-EDTA complexes in one solution have been performed. The absorptivity of the complexes at pH 2.5 and 4.9 as well as Beer's law have been studied. The content of the solution for the sample dissolving has been established. The stoichiometry of the oxidation-reduction reaction between Cu(III) from the sample and Co(II) in the solution has been proved. The possibility to dissolve the sample in air has been tested. As a result a simple and rapid spectrophotometric method for the determination of the δ non-stoichiometric oxygen coefficient in YBa 2Cu3O6.5+δ samples has been developed without precise measurement of the sample mass. The standard deviation of the δ- and y-coefficient results is 0.017. The reproducibility of the y-values in relative units (s r ) is 0.2%. The accuracy of the results has been confirmed by the method, which is based on the absorbance measurement of the I 3 - -starch compound. © 2012 Versita Warsaw and Springer-Verlag Wien.Item Spectrophotometric study of competitive complexation equilibria involving overlapped spectral responding species: Determination of the stability constant of bismuth-pyrophosphate complex(2012-12-01) Nedeltcheva T.; Surleva A.; Nikolova L.; Borissova R.; Georgieva S.Spectrophotometric study of competitive complex formation equilibria involving overlapped spectral responding species applying a simple and versatile algorithm was carried out. The algorithm involves multivariable regression for calculation of equilibrium concentrations from multiwavelength data and mass action law for the stability constant calculation. The used regression functions are part of common statistical software. Stability constants and complex stoichiometry of competing equilibria were simultaneously determined. The species concentration profiles at several spectral overlapping and α-coefficient of competing reaction were obtained. Non-absorbing bismuth - pyrophosphate (PPh) system was studied as a competitive reaction of bismuth - 4-(2-Pyridylazo) resorcinol (PAR) complex. The formation of Bi-PPh complex with 1:1 stoichiometry was proved in the studied concentration region (C Bi = 1×10 -5 mol L -1; C PPh = 5×10 -6 - 1×10 -4 mol L -1). The stability constant of the complex at pH 1 and μ= 1.0 have been determined: logβ = 4.2±0.2. © Versita Sp. z o.o.Item Spectroscopic and voltamperometric studies of Cu(II) complex with 3-amino-5,5-diphenylhydantoin(2018-01-01) Georgieva S.; Todorov P.New copper(II)-aminohydantoin complexes ([CuAPh2Cl2(OH2)2]; [CuAPh2(NH3) (OH2)2], where APh is 3-amino-5,5-diphenylhydantoin), were synthesized and analyzed by means of elemental analysis, atomic absorption spectroscopy, UV-Vis spectroscopy and voltamperometric method. The complex forming processes in the Cu(II)- aminophenytoin (APh) system are studied by differential pulse polarography (DPP). The experiments are carried out at temperature of 20±1°C in ammonia buffer solution (pH = 8.2±0.1) at ionic strength I = 0.1. The reversibility of the reduction of the peak is proved and the equations of De Ford and Hume and these of Leden to DPP data are applied. The mean ligand number n of the complexes formed is found from the slopes of the straight lines of r ΔEpr = f(lgCAPh) function. The existence of two complexes (n =1 and 2) is proved in the presence of high ligand concentration (CAPh = 2.10-4 - 3.10-3 mol l-1) and the values of its conditional and total stability constants are found. It was found that the values of the total constants are lg CuβAPh = 8.0, lgβCuAPh2 =10.9 (I = 0.1). A satisfactory good coincidence between the results obtained by the procedure proposed and the literature data is observed. The atomic absorption spectroscopy and elemental analysis confirm the compounds stoichiometry. The IR and UV/Vis spectra show that APh act as bidentate ligands with the coordination involving the carbonyl oxygen and the nitrogen atom of amino group.Item Study of Novel Peptides for Antimicrobial Protection in Solution and on Cotton Fabric(2022-08-01) Todorov P.; Georgieva S.; Staneva D.; Peneva P.; Grozdanov P.; Nikolova I.; Vasileva-Tonkova E.; Grabchev I.Some new N- and C-modified biomolecular peptide analogues of both VV-hemorphin-5 and VV-hemorphin-7 with varied amino acids (Cys, Glu, His), 1-adamantanecarboxylic acid, and niacin (nicotinic acid) were synthesized by solid-phase peptide synthesis—Fmoc (9-fluorenylmethoxy-carbonyl) chemistry and were characterized in water solutions with different pH using spectroscopic and electrochemical techniques. Basic physicochemical properties related to the elucidation of the peptide structure at physiological pH have been also studied. The results showed that the interaction of peptide compounds with light and electricity preserves the structural and conformational integrity of the compounds in the solutions. Moreover, textile cotton fibers were modified with the new compounds and the binding of the peptides to the surface of the material was proved by FTIR and SEM analysis. Washing the material with an alkaline soap solution did not show a violation of the modified structure of the cotton. Antiviral activity against the human respiratory syncytial virus (HRSV-S2) and human adenovirus serotype 5 (HAdV-5), the antimicrobial activity against B. cereus and P. aeruginosa used as model bacterial strains and cytotoxic effect of the peptide derivatives and modified cotton textile material has been evaluated. Antimicrobial tests showed promising activity of the newly synthesized compounds against the used Gram-positive and Gram-negative bacteria. The compounds C-V, H-V, AC-V, and AH-V were found slightly more active than NH7C and NCH7. The activity has been retained after the deposition of the compounds on cotton fibers.Item Synthesis of new modified with rhodamine b peptides for antiviral protection of textile materials(2021-11-01) Todorov P.; Georgieva S.; Staneva D.; Peneva P.; Grozdanov P.; Nikolova I.; Grabchev I.Here we report on the synthesis and characterization of three new N-modified analogues of hemorphin-4 with rhodamine B. Modified with chloroacetyl, chloride cotton fabric has been dyed and color coordinates of the obtained textile materials were determined. Antiviral and virucidal activities of both the peptide-rhodamine B compounds and the dyed textile material were studied. Basic physicochemical properties (acid-base behavior, solvent influence, kinetics) related to the elucidation of structural activity of the new modified peptides based on their steric open/closed ring effect were studied. The obtained results lead to the conclusion that in protic solvent with change in pH of the environment, direct control over the dyeing of textiles can be achieved. Both the new hybrid peptide compounds and the modification of functionalized textile materials with these bioactive hemorphins showed virucidal activity against the human respiratory syncytial virus (HRSV-S2) and human adenovirus serotype 5 (HAdV-5) for different time intervals (30 and 60 min) and the most active compound was Rh-3.Item Synthesis, Characterization and Biological Investigation of New N-Modified Spinorphin Analogs(2022-10-01) Todorov P.; Georgieva S.; Tchekalarova J.; Subaer S.; Peneva P.; Hartati H.The emergence of diverse peptide derivatives has been due to constant efforts to find a specific peptide with pronounced biological activity for effective application as a therapeutic. Spinorphin-peptide products have been reported to possess various applications and properties. In the present study, spinorphin peptides with a rhodamine residue and a modification in the amino acid backbone were synthesized by a solid-phase method using Fmoc chemistry. The results obtained from the spectral and electrochemical techniques used: Scanning electron microscopy (SEM), UV-vis, fluorescence, infrared spectroscopy (IR), and voltammetry were used to elucidate the structural characteristics and some physicochemical properties to gain insight into their behavior in the solid state and in aqueous solutions with different pHs. Both Rh-S5 and Rh-S6 had compound anticonvulsant effect comparable to Rh-S against psychomotor seizures at the highest dose of 20 μg. Furthermore, Rh-S6 showed a strong ability to inhibit seizure propagation and had a similar threshold to Rh-S against the intravenous pentylenetetrazol induced clonic seizure in mice; one of the three hybrid spinorphin analogs tested when screened for anticonvulsant activity. Biological tests against several bacterial pathogens such as Staphylococcus aureus, Escherichia coli, and Bacillus cereus showed similar results to negative control of the new peptide derivatives. The compounds also showed weak activity against Candida albicans fungus. The antioxidant testing results revealed more than 50% activity by reviewing the radical deterrence capabilities of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results are indicative of the ongoing search for universal antimicrobial agents with pronounced synergism when used simultaneously as anticonvulsant, antibacterial, and antifungal agents.Item Synthesis, characterization and cytotoxic activity of novel Cu(II) and Co(II) complexes with 3-amino-5, 5-dimethylhydantoin(2014-01-01) Georgieva S.; Todorov P.; Wesselinova D.Novel mixed complexes of copper (II) and cobalt (II) with 3-amino-5,5-dimethylhydantoin were synthesized and their in vitro anticancer activity was investigated. The structures of the compounds were confirmed by IR, UV-Vis spectrometry, voltammetry and elemental analysis. The cytotoxic effects of novel complexes of copper (II) and cobalt (II) with 3-amino-5,5-dimethylhydantoin were tested against a panel of human tumor cell lines. All of the compounds investigated exhibited different concentration-dependent antiproliferative effects against the HT29, MDA-MB-231, HepG2 and HeLa cell lines after 24 h of treatment. The most sensitive cells were the HepG2 cells at various concentrations of both tested compounds followed by HT29.Item Synthesis, molecular docking, electrochemical and fluorimetric analysis of new caffeic and cinnamic acid-conjugated hemorphin derivatives designed as potential anticonvulsant and antinociceptive agents(2024-02-01) Todorov P.; Georgieva S.; Peneva P.; Nikolov S.; Rangelov M.; Todorova N.; Pechlivanova D.; Tchekalarova J.Based on the pharmacophore model of opioid receptors, our team recently synthesized a series of short‐chain hemorphin peptide analogs containing non‐natural amino acids. They demonstrated anticonvulsant and antinociceptive activity with low neurotoxicity. In the present study, a series of novel bioconjugates of N-modified hemorphin analogs containing second pharmacophore cinnamic acids (CA) or caffeic (KA) were synthesized by a traditional solid-phase Fmoc chemistry method for peptide synthesis. Electrochemical and fluorimetric analysis, in vivo anticonvulsant and antinociceptive activity in mice were conducted on the compounds. The three CA acid- (H4-CA, H5-CA, and H7-CA) and three KA acid- (H4-KA, H5-KA, and H7-KA) conjugated hemorphin derivatives exhibited potency at the highest doses of 2 µg/5 µl, administered by intracerebroventricular (icv) mode, against seizure spread in the maximal electroshock test (MES) in mice. The KA-conjugated H5-KA derivate, at the lowest dose, was the only compound that suppressed clonic seizures in the subcutaneous pentylenetetrazol (scPTZ) test. Except for the H5-CA, all tested CA acid- and KA acid-conjugated peptide derivates had the potency to increase the latency for clonic seizures in a dose-dependent mode. The activity against the psychomotor seizures in the 6-Hz test was detected only for the H4-CA (0.5 µg) and H4-KA (0.5 µg and 1 µg), respectively. All investigated peptides showed a more pronounced antinociceptive effect in the “intraplantar formalin” test compared to the “hot plate” test. Shorter chain analogs showed a better antinociceptive profile against tonic pain. The data suggest a DOR and KOR-mediated mechanism of action. According to the docking analysis, H7-CA showed a different antinociceptive profile than other investigated peptides. The novel peptide derivates did not exhibit neurotoxicity in the rotarod test. Our findings suggest that conjugated CA and KA morphine peptides can be used to develop novel morphine-related analogs with anticonvulsant and antinociceptive activity.