Browsing by Author "Grozdanov P."

Now showing 1 - 7 of 7
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    Antimicrobial Properties of Chitosan-Modified Cotton Fabric Treated with Aldehydes and Zinc Oxide Particles
    (2023-07-01) Staneva D.; Atanasova D.; Angelova D.; Grozdanov P.; Nikolova I.; Grabchev I.
    Chitosan is a natural biopolymer with a proven ability to impart textile materials with antimicrobial properties when loaded onto them. The mechanism of its bacteriological activity depends on the contact between the positive and negative charges of the amino groups located on the surface of the microbes. Unfortunately, the type of microorganisms and pH influence this action–shortcomings that can be avoided by chitosan modification and by loading its film with substances possessing antimicrobial properties. In this study, chitosan was modified with benzaldehyde and crosslinked with glutaraldehyde to form a film on the surface of cotton fabric (CB). Also, another material was obtained by including zinc oxide particles (CBZ) synthesized in situ into the chitosan coating. The performed analyses (contact angle measurement, optical and scanning electron microscopy, FTIR, XRD, and thermal analysis) evidenced the modification of the cotton fabric and the alteration of the film properties after zinc oxide inclusion. A comparison of the antimicrobial properties of the new CB with materials prepared with chitosan without benzaldehyde from our previous study verified the influence of the hydrophobicity and surface roughness of the fabric surface on the enhancement of antimicrobial activity. The microbial growth inhibition increased in the following order: fungal strain Candida lipolytica >Gram-positive bacteria Bacillus cereus >Gram-negative bacteria Pseudomonas aeruginosa. The samples containing zinc oxide particles completely inhibited the growth of all three model strains. The virucidal activity of the CB was higher against human adenovirus serotype 5 (HAdV-5) than against human respiratory syncytial virus (HRSV-S2) after 60 min of exposure. The CBZ displayed higher virucidal activity with a Δlog of 0.9 against both viruses.
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    Hyperbranched polymers modified with Dansyl units and their Cu(II) complexes. bioactivity studies
    (2020-10-02) Bosch P.; Staneva D.; Vasileva-Tonkova E.; Grozdanov P.; Nikolova I.; Kukeva R.; Stoyanova R.; Grabchev I.
    Two new copper complexes of hyperbranched polymers modified with dansyl units were synthesized and characterized by infrared spectroscopy (IR) and electron paramagnetic resonance (EPR) techniques. It was found that copper ions coordinate predominantly with nitrogen or oxygen atoms of the polymer molecule. The place of the formation of complexes and the number of copper ions involved depend on the chemical structure of the polymer. The antimicrobial activity of the new polymers and their Cu(II) complexes was tested against Gram-negative and Gram-positive bacterial and fungal strains. Copper complexes were found to have activity better than that of the corresponding ligands. The deposition of the modified branched polymers onto cotton fabrics prevents the formation of bacterial biofilms, which indicates that the studied polymers can find application in antibacterial textiles.
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    Metal–Peptide Complexes with Antimicrobial Potential for Cotton Fiber Protection
    (2023-02-01) Georgieva S.; Todorov P.; Staneva D.; Grozdanov P.; Nikolova I.; Grabchev I.
    A study of the formation of copper (II) complexes with hemorphin peptide motifs in alkalic water solutions is presented. The effect of the peptide ligand on the complexing properties of the Cu (II) ion was quantified by giving the stoichiometry and stability of the complex compounds in the medium in which they are formed using voltammetric (cyclic) and spectral (UV-Vis and fluorimetric) analytical techniques. The resulting complexes were examined via IR spectroscopy to detect M-N and M-O oscillations and using the EPR approach in solution and in the solid phase to view the coordination and ligand binding regime. The possibility of the synergistic action of copper ions in the antivirus protection processes of cotton fibers coated in the same solvent with the newly obtained complex compounds was also investigated. One of the advantages is the formation of the complexes in an environment where the immobilization takes place, which contributes to increasing the efficiency of the process. The obtained results may serve as an aid for future more detailed biological studies of structure–activity relationships (SARs).
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    New Poly(Propylene Imine) dendrimer modified with acridine and its Cu(II) complex: Synthesis, characterization and antimicrobial activity
    (2019-09-01) Bosch P.; Staneva D.; Vasileva-Tonkova E.; Grozdanov P.; Nikolova I.; Kukeva R.; Stoyanova R.; Grabchev I.
    A second-generation poly(propylene imine) dendrimer modified with acridine and its Cu(II) complex have been synthesized for the first time. It has been found that two copper ions form complexes with the nitrogen atoms of the dendrimeric core by coordinate bonds. The new compounds have been characterized by nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), fourier-transform infrared spectroscopy (FTIR) and fluorescence spectroscopy. The spectral characteristics of the modified dendrimer have been measured in different organic solvents, and a negative fluorescence solvatochromism has been observed. The antimicrobial activity of the dendrimers has been tested against model pathogenic microorganisms in agar and by broth dilution method. The cotton fabric treated with both dendrimers has been evaluated towards pathogenic microorganisms. The obtained modified cotton fabrics have been shown to hamper bacterial growth and to prevent biofilm formation. Dendrimer cytotoxicity has been investigated in vitro in the model HEp-2 cell line.
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    Study of Novel Peptides for Antimicrobial Protection in Solution and on Cotton Fabric
    (2022-08-01) Todorov P.; Georgieva S.; Staneva D.; Peneva P.; Grozdanov P.; Nikolova I.; Vasileva-Tonkova E.; Grabchev I.
    Some new N- and C-modified biomolecular peptide analogues of both VV-hemorphin-5 and VV-hemorphin-7 with varied amino acids (Cys, Glu, His), 1-adamantanecarboxylic acid, and niacin (nicotinic acid) were synthesized by solid-phase peptide synthesis—Fmoc (9-fluorenylmethoxy-carbonyl) chemistry and were characterized in water solutions with different pH using spectroscopic and electrochemical techniques. Basic physicochemical properties related to the elucidation of the peptide structure at physiological pH have been also studied. The results showed that the interaction of peptide compounds with light and electricity preserves the structural and conformational integrity of the compounds in the solutions. Moreover, textile cotton fibers were modified with the new compounds and the binding of the peptides to the surface of the material was proved by FTIR and SEM analysis. Washing the material with an alkaline soap solution did not show a violation of the modified structure of the cotton. Antiviral activity against the human respiratory syncytial virus (HRSV-S2) and human adenovirus serotype 5 (HAdV-5), the antimicrobial activity against B. cereus and P. aeruginosa used as model bacterial strains and cytotoxic effect of the peptide derivatives and modified cotton textile material has been evaluated. Antimicrobial tests showed promising activity of the newly synthesized compounds against the used Gram-positive and Gram-negative bacteria. The compounds C-V, H-V, AC-V, and AH-V were found slightly more active than NH7C and NCH7. The activity has been retained after the deposition of the compounds on cotton fibers.
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    Synthesis of new modified with rhodamine b peptides for antiviral protection of textile materials
    (2021-11-01) Todorov P.; Georgieva S.; Staneva D.; Peneva P.; Grozdanov P.; Nikolova I.; Grabchev I.
    Here we report on the synthesis and characterization of three new N-modified analogues of hemorphin-4 with rhodamine B. Modified with chloroacetyl, chloride cotton fabric has been dyed and color coordinates of the obtained textile materials were determined. Antiviral and virucidal activities of both the peptide-rhodamine B compounds and the dyed textile material were studied. Basic physicochemical properties (acid-base behavior, solvent influence, kinetics) related to the elucidation of structural activity of the new modified peptides based on their steric open/closed ring effect were studied. The obtained results lead to the conclusion that in protic solvent with change in pH of the environment, direct control over the dyeing of textiles can be achieved. Both the new hybrid peptide compounds and the modification of functionalized textile materials with these bioactive hemorphins showed virucidal activity against the human respiratory syncytial virus (HRSV-S2) and human adenovirus serotype 5 (HAdV-5) for different time intervals (30 and 60 min) and the most active compound was Rh-3.
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    Synthesis, Spectral Characteristics, Sensing Properties and Microbiological Activity of New Water-Soluble 4-Sulfo-1,8-naphthalimides
    (2024-05-01) Said A.I.; Staneva D.; Vasileva-Tonkova E.; Grozdanov P.; Nikolova I.; Stoyanova R.; Jordanova A.; Grabchev I.
    A new water-soluble polyamidoamine (PAMAM) dendrimer modified with 4-sulfo-1,8-naphthalimide (DSNI) and its monomeric structural analogue (MSDI) were synthesized. Their photophysical properties were investigated in organic solvents of different polarities and aqueous solutions. The effect of pH on fluorescence intensity was determined. It was found that the dendrimer emits blue fluorescence in an acidic medium, which is quenched in an alkaline environment. This phenomenon is due to the possibility of suppression of nonradiative photoinduced electron transfer in acidic media. The influence of different metal ions (Cu2+, Pb2+, Sn2+, Sr2+, Mg2+, Ba2+, Co2+, Hg2+, Zn2+, Ni2+, Fe3+, Al3+) and anions (CN−, S2−, S2O52−, HPO42−, H2PO4−, F−, CH3COO−, NO2−, CO32−, SO42−) on the intensity of the emitted fluorescence was studied. Quenching was only found in the presence of Cu2+. This makes the dendrimer suitable for determining copper ions in water solutions in the presence of other metal ions and anions. Additionally, DSNI was used as a ligand to obtain a stable copper complex, the structure of which was investigated by electron paramagnetic resonance (EPR), infrared spectrum, and elemental analysis. Two copper ions were found to form a complex with one dendrimer. The in vitro microbiological activity of the new compounds against bacteria Pseudomonas aeruginosa and two viruses HRSV-2 and HAdV-5 was investigated. With a view to obtaining antibacterial and anti-viral textiles, cotton fabrics were treated with the three compounds, and then their activity against the same microbial strains was investigated. It was found that the microbiological activity was preserved after the application of the new compounds to the cotton fabrics.