Browsing by Author "Laiche A.T."

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    Effect of Juniperus communis extract on probiotic properties of Bacillus safensis isolated from camel milk in the region of El Oued (Algeria)
    (2024-01-01) Layadi I.; Laiche A.T.; Tlili M.L.; Messaoudi M.; Ghemam Amara D.; Mezghani-Khemakhem M.; Naccache C.; Sawicka B.; Atanassova M.; Zahnit W.; Ahmad S.F.
    The current study focuses on the effect of Juniperus communis extract on the probiotic properties of lactic acid bacteria isolated from camel milk in the region of El Oued (Algeria). Chromatographic analysis by HPLC was carried out to detect the most important compounds extracted from the plant. The total phenolic and flavonoid contents were determined using the colorimetric procedures Folin–Ciocalteu and aluminum chloride. The probiotic properties were studied and evaluated in vivo with Juniperus communis extracts after isolating strains from camel's milk and identifying them using 16S rRNA gene sequencing. Chromatographic profiles of the phenolic compounds revealed that Juniperus communis extract is rich in quercetin. After conducting chemical analyses of polyphenols and flavonoids, the results demonstrated a high content of phenolic compounds in Juniperus communis extracts (polyphenols: 103.80 ± 0.30 mg GAE/g E. flavonoids: 15.85 ± 0.80 mg QE/g E). Sequencing and phylogenetic analysis showed that the isolates belong to Bacillus pumilus and Bacillus safensis strains. The combination of Juniperus communis and Bacillus safensis restored the healthy intestine wall structure and returned the blood biochemical parameters to normal values. It was found that the mixture enhanced anti-inflammatory effectiveness by reducing erythrocyte sedimentation rate and C-reactive protein values. Juniperus communis has a high polyphenol and flavonoid content which can have a considerable impact on Bacillus safensis probiotic properties.