Browsing by Author "Uzunova V."

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    BIOCOMPATIBILITY AND ANTIFUNGAL ACTIVITY OF SILVER DOPED SiO2/PECTIN COMPOSITE MATERIALS
    (2021-01-01) Angelova T.; Uzunova V.; Rangelova N.; Georgieva N.; Momchilova A.; Tzoneva R.
    The purpose of the study was to evaluate the biocompatibility and antifungal activity of silver doped SiO2/citrus pectin composite materials. The effect of the amount of silver content (0 - 2.5 wt. %) on biocompatibility using L929 fibroblast cells and antifungal activity against Candida albicans 74 was observed. It was established that composites containing 0.5 wt. % and 1 wt. % Ag exhibited biocompatibility as well as revealed low cytotoxicity, good cell adhesion and spreading and luck of apoptosis. The antifungal activity of the composites demonstrated that the size of formed inhibition zones depends on the amount of silver in the materials. Based on the obtained results, silver doped SiO2/ pectin composite materials were proven to be efficient for use as in vivo implants in tissue engineering applications.
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    Cytotoxicity and antibiofilm activity of SiO2/cellulose derivative hybrid materials containing silver nanoparticles
    (2016-01-01) Angelova T.; Rangelova N.; Uzunova V.; Georgieva N.; Andreeva T.; Momchilova A.; Tzoneva R.; Müller R.
    Hybrid materials based on tetraethyl orthosilicate (TEOS), hydroxypropyl cellulose (HPC), or hydroxypropyl methyl cellulose (HPMC) with silver nanoparticles were synthesized. They were analyzed and characterized using differential thermal analysis and thermogravimetry, atomic force microscopy, and static contact angle measurements. It was experimentally demonstrated that the silverdoped hybrid materials have pronounced antibacterial behavior by studying the reduction of Pseudomonas aeruginosa PAO1 biofilm formation on the tested materials. The results revealed biofilm reduction of 35.7% and 30% by SiO2/HPC/2.5% Ag and SiO2/HPMC/2.5% Ag hybrid materials, respectively, compared to the control. Cytotoxicity of examined materials and actin cytoskeleton organization of fibroblasts seeded on the materials was studied as a function of material properties as the type of surface functional groups and silver content. The obtained hybrid materials with low silver content proved efficient in tissue engineering applications since they showed good antibacterial and noncytotoxic properties for eukaryotic cells.