Browsing by Author "Vetskov N."

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    COMPARATIVE STUDY OF POLYPHENOLIC COMPOSITION AND REDUCING PROPERTIES OF EXTRACTS OF CANNABIS SATIVA L., CANNABIS INDICA AND CANNABIS HYBRID
    (2024-01-01) Vetskov N.; Kaloyanov N.; Hinkov I.; Diankov S.; Pashova K.; Gerasimova V.; Funeva-Peycheva M.; Yankovska-Stefanova T.; Garbev A.
    Cannabis Sativa L., Cannabis Indica and Cannabis Hybrid are widespread plant species that find legal use in many countries around the world. The first of them has found considerable application in medicine, cosmetics, building construction, textile and food industries. Cannabis Indica and Cannabis Hybrid are used as raw materials for the extraction of oils that are used as pain relievers as well as for recreation purposes. The aim of the present study is to compare the polyphenolic composition of extracts from the three types of plants, by the maceration method of dry green leaf mass consisting of inflorescences and leaves. Ethanol (99.9 %) is used as an extractant, and the duration of the maceration process is seven days, at room temperature and stirring. The polyphenol composition is determined using HPLC-DAD method. The following polyphenol composition is found in extracts of Cannabis Sativa L.: epigallocatechin, rutin, myricetin, quercetin and kaempferol. On the other hand, quercetin and kaempferol are detected in the Cannabis Indica samples, while epigallocatechin, quercetin and kaempferol are present in the hybrid sort. In addition, green synthesis of silver nanoparticles by using sunlight or microwave irradiation was carried out to evaluate the reduction properties of three cannabis extracts. The least quantity of silver nanoparticles was obtained in the presence of Cannabis Sativa L. extracts. From the other two species of cannabis, microwave irradiation produces more silver nanoparticles, with smaller sizes, while exposure to direct sunlight produces fewer particles, but with larger sizes.
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    THE EFFECT OF RADIATION PRESERVATION ON MACROMOLECULE CONTENT, ANTIMICROBIAL ACTIVITY AND RADICAL - SCAVENGING POTENTIAL OF PORTULACA OLERACEA L. LEAVES EXTRACT
    (2024-01-01) Karamalakova Y.; Petkova-Parlapanska K.; Hinkov I.; Vetskov N.; Georgieva E.; Nikolova G.
    The Portulaca oleracea L. (P. oleracea) phytochemical composition (contains omega-3 fatty acids, quercetin, rutin, gallo tannins, proteins) is responsible for its biological effects, e.g. antioxidative, antibacterial, anti-inflammatory and antiradiation activity. The plant has a C4 metabolism and it is known for its tolerance to different stressors, such as salinity, no water, high - temperature conditions, and 9 kGy radiation. The purpose of the present investigation was focused on the inhibitory effects of P. oleracea leaves extracts against 5 - 10 kGy radiation - induced abiotic stress, and assess to total phenol, flavonoid, and tannins content; antibacterial potential (Staphylococcus aureus ATCC25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) and antioxidant capacity. The free dried, powdered P. oleracea samples were 60Co irradiated, 50 % ethanol solvent (v/v) extracted and compared to 0 kGy extract. 10 kGy irradiated extract provide to be potentially effective against bacterial strains and possessed stable antioxidant activity, towards DPPH (p ˂ 0.002), ABTS•+ (p ˂ 0.05), FRAP (p ˂ 0.05) and NO (p ˂ 0.002). In addition, highly sensitive Electron Paramagnetic Resonance (EPR) was used to evaluate antiradical capacity. Single, symmetrical signal (g = 2.0023) was recorded in 10 kGy irradiated P. oleracea extract, comparable to the EPR signal in non-irradiated sample and depended directly on the antiradical potential. Moreover, in vitro P. oleracea inhibited the superoxide anion (•O2 −), hydroxyl (•OH), alkyl radicals, and exhibited antioxidant properties against 10 kGy irradiation.