SYNTHESIS AND STABILITY OF A RAPANA THOMASIANA HEMOCYANIN CONJUGATED WITH VITAMIN B9

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creativework.keywordsDsc, Ftir, Rapana thomasiana hemocyanin, Secondary structure, Thermal stability, Vitamin b9
creativework.publisherUniversity of Chemical Technology and Metallurgyen
dc.contributor.authorGuncheva M.
dc.contributor.authorRaynova Y.
dc.contributor.authorIdakieva K.
dc.contributor.authorTodinova S.
dc.contributor.authorYancheva D.
dc.date.accessioned2024-07-16T11:16:51Z
dc.date.accessioned2024-07-16T11:19:28Z
dc.date.available2024-07-16T11:16:51Z
dc.date.available2024-07-16T11:19:28Z
dc.date.issued2020-01-01
dc.description.abstractThis is the first report on the synthesis of vitamin B9-Rapana thomasiana hemocyanin conjugates (vit. B9-RtH). Proteins modified with 25 and 150 vitamin B9 residues аre obtained in a coupling reaction between N-hydroxysuccinimide-activated γ -carboxylic groups of vitamin B9 and the amino-groups of N-terminal amino acids and lysines comprising the protein. ATR-FTIR spectroscopy is applied to monitor the changes of the secondary structure upon the modification. The comparison of the secondary structure of the native and the vitamin B9-conjugated RtHs shows that the modification results in an increase of the β-structures in a favor of α-helices and coiled structures. The therrmal stability of the novel preparations is evaluated by differential scanning calorimetry (DSC). Despite the serious changes of the conformation, the vit.-B9-RtH conjugates are characterized by a high thermal stability. It is assumed that the changes of the DSC profiles are due to alternative protein-protein interactions, e.g H-bonding, stacking or hydrophobic interactions with vitamin B9 moieties that are possible in the modified RtH molecules
dc.identifier.issn1314-7978
dc.identifier.issn1314-7471
dc.identifier.scopusSCOPUS_ID:85129276447en
dc.identifier.urihttps://rlib.uctm.edu/handle/123456789/1379
dc.language.isoen
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85129276447&origin=inward
dc.titleSYNTHESIS AND STABILITY OF A RAPANA THOMASIANA HEMOCYANIN CONJUGATED WITH VITAMIN B9
dc.typeArticle
oaire.citation.issue2
oaire.citation.volume55
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