Structural, thermal, and storage stability of rapana thomasiana hemocyanin in the presence of cholinium-amino acid-based ionic liquids

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creativework.keywordsAntibacterial activity, Antibiofilm formation, DSC, FTIR, Hemocyanin, Ionic liquids, Storage stability
creativework.publisherMDPI AGen
dc.contributor.authorGuncheva M.
dc.contributor.authorIdakieva K.
dc.contributor.authorTodinova S.
dc.contributor.authorYancheva D.
dc.contributor.authorPaunova-Krasteva T.
dc.contributor.authorOssowicz P.
dc.contributor.authorJanus E.
dc.date.accessioned2024-07-10T14:27:04Z
dc.date.accessioned2024-07-10T14:49:40Z
dc.date.available2024-07-10T14:27:04Z
dc.date.available2024-07-10T14:49:40Z
dc.date.issued2021-03-02
dc.description.abstractNovel biocompatible compounds that stabilize proteins in solution are in demand for biomedical and/or biotechnological applications. Here, we evaluated the effect of six ionic liquids, containing mono-or dicholinium [Chol]1or2 cation and anions of charged amino acids such as lysine [Lys], arginine [Arg], aspartic acid [Asp], or glutamic acid [Glu], on the structure, thermal, and storage stability of the Rapana thomasiana hemocyanin (RtH). RtH is a protein with huge biomedicinal potential due to its therapeutic, drug carrier, and adjuvant properties. Overall, the ionic liquids (ILs) induce changes in the secondary structure of RtH. However, the structure near the Cu-active site seems unaltered and the oxygen-binding capacity of the protein is preserved. The ILs showed weak antibacterial activity when tested against three Gram-negative and three Gram-positive bacterial strains. On the contrary, [Chol][Arg] and [Chol][Lys] exhibited high anti-biofilm activity against E. coli 25213 and S. aureus 29213 strains. In addition, the two ILs were able to protect RtH from chemical and microbiological degradation. Maintained or enhanced thermal stability of RtH was observed in the presence of all ILs tested, except for RtH-[Chol]2 [Glu].
dc.identifier.doi10.3390/molecules26061714
dc.identifier.issn1420-3049
dc.identifier.scopusSCOPUS_ID:85103863003en
dc.identifier.urihttps://rlib.uctm.edu/handle/123456789/640
dc.language.isoen
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85103863003&origin=inward
dc.titleStructural, thermal, and storage stability of rapana thomasiana hemocyanin in the presence of cholinium-amino acid-based ionic liquids
dc.typeArticle
oaire.citation.issue6
oaire.citation.volume26
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