A Way to Immobilization of Antimicrobial Peptidenisin on Polydimethylsiloxane Surface
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2025-01-01
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Abstract: Medical devices associated infections due to microbial attachment and biofilm formation are with a high impact on human health and huge socioeconomic costs. Increasing resistance to traditional antibiotics and multidrug treatments are already recognized as one of the top-most serious threats to human health. This rises an argent need in the development of new antimicrobial agents, materials and strategies for improved protection of medical devices against infections. Immobilization of antimicrobial peptides onto the material surface is one of them. Many medical devices are fabricated by chemically inert polymers, like polydimethylsiloxane (PDMS), polystyrene, polyethylene, etc. to which surfaces is difficult the chemical bonding of biomolecules. The aim of this investigation is to demonstrate the ability of plasma-based Ar+ beam (PBAIB) to initiate antimicrobial peptides immobilization onto the chemically inert PDMS surface, using the bacteriocin nisin as an example. Earlier developed by us multi-step procedure was utilized that makes possible three types bonding of antimicrobial peptides: i) linker - free, at the first step, just after the PBAIB treatment; ii) via vinyl monomer linker, at the second step after grafting of vinyl monomer and iii) via flexible spacer after coupling of di-NH2PEG on vinyl monomer grafted surface. A parallel plate reactor, equipped with a serial capacitance, was employed to ensure arise of an ion flow inside the plasma volume, directed toward the treated sample. The changes in the chemical composition of the PDMS surface were studied at every step of the modification procedure and the successful immobilisation of nisin via flexible spacer (di-NH2-PEG5000) was proved by XPS analysis. This multi-step procedure to biofunctionalization of strong hydrophobic chemically inert polymer surfaces has a potential to be used whenever need arises to control antimicrobial activity of PDMS or other chemically inert polymeric materials and medical devices fabricated by them.