Production and chracterization of lipase from pseudozyma antarctica NBIMCC 8340

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creativework.keywordsLipase, Lipase activity, Lipase optimums, Pseudozyma antarctica, Pseudozyma antarctica cultivation
creativework.publisherUniversity of Chemical Technology and Metallurgyjournal@uctm.eduen
dc.contributor.authorBorisov B.
dc.contributor.authorManova D.
dc.contributor.authorMarinkova D.
dc.contributor.authorYotova L.
dc.contributor.authorDanalev D.
dc.date.accessioned2024-07-16T11:16:47Z
dc.date.accessioned2024-07-16T11:18:22Z
dc.date.available2024-07-16T11:16:47Z
dc.date.available2024-07-16T11:18:22Z
dc.date.issued2015-01-01
dc.description.abstractLipases are serine hydrolases, defined as triacyl-glycerol acylhydrolases. They catalyze both hydrolytic and synthetic reactions showing substrate, regio- and stereo selectivity. The psyhrophilic strain Pseudozyma antarctica, isolated in the cold conditions of the Antarctica, can produce a thermostable extracellular lipase. In this work we report the synthesis of an extracellular lipase from Pseudozyma antarctica. The strain was cultured in flasks and various parameters were monitored during the proliferation process. Several cultivation procedures were done in order to find optimal conditions. Further, activity of the crude lipase, its temperature and pH optimum were determined using a potentiometric method. We found temperature optimum at 60°C and pH optimum at pH 8.0. The determined specific activity of the crude lipase was 97,2 U/mg. Activity and protein content of the ``crude`` post cultivation liquid were also determined.
dc.identifier.issn1314-7978
dc.identifier.issn1314-7471
dc.identifier.scopusSCOPUS_ID:84944321407en
dc.identifier.urihttps://rlib.uctm.edu/handle/123456789/1117
dc.language.isoen
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84944321407&origin=inward
dc.titleProduction and chracterization of lipase from pseudozyma antarctica NBIMCC 8340
dc.typeArticle
oaire.citation.issue5
oaire.citation.volume50
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