Phenotypic characterization and molecular identification of Bacillus pumilus isolated from Algerian goat’s milk and evaluation of its synbiotic effect with Hammada scoparia L. extract and ZnO-NPs

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creativework.keywords16S rRNA, Goat’s milk, H. scoparia, prebiotic activity, probiotics, ZnO-NPs
creativework.publisherTaylor and Francis Ltd.en
dc.contributor.authorChaima B.
dc.contributor.authorBoutlelis D.A.
dc.contributor.authorTouhami L.A.
dc.contributor.authorGhemam Amara D.
dc.contributor.authorKhemakhem M.M.
dc.contributor.authorNaccache C.
dc.contributor.authorAtanassova M.
dc.contributor.authorZahnit W.
dc.contributor.authorAhmad S.F.
dc.contributor.authorMessaoudi M.
dc.date.accessioned2024-09-02T14:58:15Z
dc.date.accessioned2024-09-02T15:15:27Z
dc.date.available2024-09-02T14:58:15Z
dc.date.available2024-09-02T15:15:27Z
dc.date.issued2024-01-01
dc.description.abstractThis study was conducted to identify and characterize the Bacillus strain from Goat’s milk in Algeria and to evaluate the prebiotic effect of H. scoparia aqueous extract and ZnO-NPs on the isolated Bacillus strain. Potentially probiotic bacteria were isolated and identified based on phenotypic characteristics, including the morphological, physiological (growth temperature, salt tolerance), biochemical (API 10S strip kits), and confirmed by 16S rDNA gene sequencing. Bacillus pumilus specie of lactic acid bacteria were identified. The base pair length of amplified DNA for the isolate was 1500 Bp. The isolates could not grow at 10°C and 45°C and tolerate up to 6% salt. H. scoparia extract and ZnO-NPs have a beneficial effect on the Bacillus pumilus strain for acid tolerance, bile tolerance, and cell surface hydrophobicity respectively; H. scoparia extract and ZnO-NPs results are high comparing to the control group’s results. As the concentration of the tested samples increases, the percentage of probiotic characteristics of Bacillus pumilus increases, in contrast to the control sample. Therefore, they could be useful tools to define adequate combinations in colonic foods development and health benefits. The beneficial physiological and biochemical properties of isolated Bacillus pumilus space from Goat’s milk with the H. scoparia aqueous extract and ZnO-NPs revealed their potential applications in the food industry.
dc.identifier.doi10.1080/10942912.2024.2391399
dc.identifier.issn1532-2386
dc.identifier.issn1094-2912
dc.identifier.scopusSCOPUS_ID:85202162800en
dc.identifier.urihttps://rlib.uctm.edu/handle/123456789/1469
dc.language.isoen
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85202162800&origin=inward
dc.titlePhenotypic characterization and molecular identification of Bacillus pumilus isolated from Algerian goat’s milk and evaluation of its synbiotic effect with Hammada scoparia L. extract and ZnO-NPs
dc.typeArticle
oaire.citation.issue1
oaire.citation.volume27
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