Xenon decreases cell migration and secretion of a pro-angiogenesis factor in breast adenocarcinoma cells: Comparison with sevoflurane

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creativework.keywordsanaesthetics sevoflurane; anaesthetics xenon, cancer angiogenesis, cancer breast, cell function migration
creativework.publisherOxford University Pressen
dc.contributor.authorAsh S.A.
dc.contributor.authorValchev G.I.
dc.contributor.authorLooney M.
dc.contributor.authorNi Mhathuna A.
dc.contributor.authorCrowley P.D.
dc.contributor.authorGallagher H.C.
dc.contributor.authorBuggy D.J.
dc.date.accessioned2024-07-10T14:27:03Z
dc.date.accessioned2024-07-10T14:48:05Z
dc.date.available2024-07-10T14:27:03Z
dc.date.available2024-07-10T14:48:05Z
dc.date.issued2014-01-01
dc.description.abstractBackground While volatile agents have been implicated in metastasis-enhancing effects on cancer cells, the effects of xenon are unknown. We investigated xenon-and sevoflurane-mediated effects on migration and expression of angiogenesis biomarkers in human breast adenocarcinoma cells. Methods MDA-MB-231 and MCF-7 cells were exposed to xenon 70% with O2 25%, CO2 5%; control gas containing O2 25%, CO2 5%, N2 70%; or sevoflurane 2.5 vol% administered in O2 60%, N2 37%, or control gas. Cell viability was determined by the MTT assay. Migration at 24 h was determined using the Oris™ Cell Migration Assay. Secretion of angiogenesis factors was measured using a membrane-based immunoassay array. Results Xenon reduced MDA-MB-231 migration to 59 (13%) after 1-h exposure (P=0.02), 64 (10%) after 3 h (P=0.01), and 71 (9%) after 5 h (P=0.04) compared with control gas, without affecting viability. Similarly, MCF-7 migration was significantly reduced at all timepoints [to 58 (12%) at 1 h, 65 (12%) at 3 h, and 65% (12%) at 5 h]. Sevoflurane did not affect migration when delivered in control gas. Glycine, an N-methyl-d-aspartate receptor co-agonist, antagonized the effects of xenon on migration. Expression of the pro-angiogenesis factor regulated on activation, normal T cell expressed and secreted (RANTES) was reduced in conditioned medium from xenon-exposed MDA-MB-231 cells compared with cells exposed to either control gas or sevoflurane [mean dot density 2.0 (0.2) compared with 3.0 (0.1) and 3.1 (0.3), respectively (P=0.02)]. Conclusion Xenon, but not sevoflurane, inhibited migration in both oestrogen receptor positive and negative breast adenocarcinoma cells. Furthermore, xenon decreased release of the pro-angiogenic factor RANTES from MDA-MB-231 cells. © 2014 The Author.
dc.identifier.doi10.1093/bja/aeu191
dc.identifier.issn1471-6771
dc.identifier.issn0007-0912
dc.identifier.scopusSCOPUS_ID:84904966132en
dc.identifier.urihttps://rlib.uctm.edu/handle/123456789/315
dc.language.isoen
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904966132&origin=inward
dc.titleXenon decreases cell migration and secretion of a pro-angiogenesis factor in breast adenocarcinoma cells: Comparison with sevoflurane
dc.typeArticle
oaire.citation.issueSUPPL. 1
oaire.citation.volume113
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