Preparation of chitosan beads for trypsin immobilization

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creativework.keywordsChitosan beads, Trypsin, Trypsin immobilization
creativework.publisherDiagnosis Press Limited.diagnosis@ibn.bgen
dc.contributor.authorKamburov M.
dc.contributor.authorLalov I.
dc.date.accessioned2024-07-10T14:27:03Z
dc.date.accessioned2024-07-10T14:48:30Z
dc.date.available2024-07-10T14:27:03Z
dc.date.available2024-07-10T14:48:30Z
dc.date.issued2014-04-16
dc.description.abstractThe present study deals with the potential application of chitosan macrobeads for immobilization of trypsin. Macrobeads were prepared by precipitation and their mechanical properties were improved by covalent crosslinking using glutaraldehyde. Scanning electron microscopy studies showed that the beads size varied between 2.5 mm and 3.5 mm depending on the chitosan content and molar excess of glutaraldehyde. Trypsin (EC.3.4.21.4) was chosen as model enzyme for immobilization on the chitosan gel beads pre-activated with glutaraldehyde. Catalytic properties and kinetic parameters of free and immobilized trypsin were determined and the impact of various technological parameters on enzyme activity was investigated. The obtained results showed that the optimal pH and temperature for the hydrolytic reaction catalyzed by immobilized trypsin for low molecular weight substrates were pH 8.0 and 50oC, respectively. The effect of substrate concentration was also studied and the values of the constants thus determined were: Km=1,54 mM/L and Vmax – 3.3 μM/min.
dc.identifier.doi10.5504/50YRTIMB.2011.0029
dc.identifier.issn1310-2818
dc.identifier.scopusSCOPUS_ID:84949781782en
dc.identifier.urihttps://rlib.uctm.edu/handle/123456789/359
dc.language.isoen
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84949781782&origin=inward
dc.titlePreparation of chitosan beads for trypsin immobilization
dc.typeArticle
oaire.citation.volume26
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