Co-immobilization of peroxidase and tyrosinase onto hybrid membranes obtained by the sol-gel method for the construction of an optical Biosensor

Simple item page
creativework.keywordsMultienzyme systems, Optical biosensor, Peroxidase, Sol-gel, Tyrosinase
dc.contributor.authorYotova L.
dc.contributor.authorYaneva S.
dc.contributor.authorMarinkova D.
dc.contributor.authorSerfaty S.
dc.date.accessioned2024-07-10T14:27:03Z
dc.date.accessioned2024-07-10T14:48:01Z
dc.date.available2024-07-10T14:27:03Z
dc.date.available2024-07-10T14:48:01Z
dc.date.issued2013-09-04
dc.description.abstractThe sol-gel method was used to synthesise two groups of hybrid membranes from silica precursors: ETMS (ethyltrimethoxysilane) and MTES (methyltriethoxysilane), and cellulose derivatives. Peroxidase isolated from horseradish and tyrosinase isolated from mushrooms was immobilized by covalent immobilization. The kinetic parameters of the isolated enzymes, pH and temperature optimum were studied. The results of this study showed a higher relative and residual activity of the enzyme when immobilized on membranes containing cellulose acetate propionate with high molecular weight CAP/H. Membranes with the best parameters were used as carriers of the multienzyme peroxidase-tyrosinase system. An optical biosensor with an oxygen electrode was constructed. Potentially, the sensor could be used for monitoring of the environment, detecting phenols in wastewater, food stuff, and in medicine.
dc.identifier.doi10.5504/BBEQ.2013.0005
dc.identifier.issn1310-2818
dc.identifier.scopusSCOPUS_ID:84883260191en
dc.identifier.urihttps://rlib.uctm.edu/handle/123456789/282
dc.language.isoen
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84883260191&origin=inward
dc.titleCo-immobilization of peroxidase and tyrosinase onto hybrid membranes obtained by the sol-gel method for the construction of an optical Biosensor
dc.typeArticle
oaire.citation.issue3
oaire.citation.volume27
Files
Collections
Articles