IN VITRO ASSESSMENT OF LACTOBACILLUS SPP. TO GROW IN THE PRESENCE OF DIFFERENT CARBON SOURCES IN THE MEDIUM
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2022-01-01
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The selection of suitable media plays a central role in attempts to improve the production of biomass from lactobacilli and the yield of products during fermentation. For this purpose, the growth capacity of 41 candidate probiotic lactobacilli from katak, yoghurt and white-brined and yellow cheese in the presence of different carbon sources in the medium was investigated. To access their abilities, in vitro model systems based on modified MRS broth, containing different carbon sources (2 % and 5 % (w/v) lactose or 2 % (w/v) glucose, prebiotics (inulin, galactooligosaccharides, glucooligosaccharides and fructooligosaccharides) and 30 % (w/v) sucrose) were designed. It was established that Lactiplantibacillus plantarum S8 and S10 had the highest growth rate in modified MRS with 2 % (w/v) glucose, whereas Lactobacillus delbrueckii subsp. bulgaricus Ro34 showed the highest growth rate in modified MRS broth with 5 % (w/v) lactose. In the presence of modified MRS, containing 20 % (w/v) prebiotics, most Lactobacillus strains showed complete utilisation towards galactooligosaccharides, followed by inulin, fructooligosaccharides and glucooligosaccharides. Seventy-three per cent of strains could grow in the presence of 30 % (w/v) sucrose in the medium and were determined as possible exopolysaccharide producers. In addition, the biofilm-formation capacity of the lactobacilli, cultivated in these media, was tested. Lactiplantibacillus plantarum OC1 and BS32 demonstrated good biofilm-formation in the presence of galactooligosaccharides, whereas Ligilactobacillus salivarius subsp. salicinus KC2 formed stable biofilms in the presence of galactooligosaccharides and fructooligosaccharides.