Optimization of extraction of antioxidants from Bulgarian Mavrud by-Products
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2018-01-01
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Abstract
Grape pomace, an abundant by-product from the wine industry, is released in large quantities but remains unused. This investigation is focused on the optimization of extraction from grape pomace and grape seeds discharged as wastes from the Bulgarian Mavrud winery. The optimized parameters refer to the solid to the solvent ratio effect (1/10 g mL-1, 1/20 g mL-1, 1/30 g mL-1, 1/40 g mL-1, 1/50 g mL-1) and the solvent concentration (water or aqueous ethanol solutions) in relation on the total polyphenol, flavonoid and anthocyanin contents. The Trolox equivalent antioxidant capacity is also determined. All analyses are performed spectrophotometrically. All extraction runs are carried out at constant extractive parameters chosen previously - temperature, sample particle size, extraction manner. The optimal conditions are selected for the Mavrud seeds and pomace - 50 % ethanol in water solution, 1:20 g ml-1 solid to solvent ratio. The selection of the best extraction conditions is of great importance because it could alter the characteristics of the final extract having an economic impact on the extraction evaluation of the Bulgarian bio-Mavrud by-products.