Determination of organophosphorus pesticides in liver samples using an optical biosensor
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2019-01-01
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Abstract
Biosensors are a promising alternative of the existing chromatographic methods as GC, HPLC, etc. They are fast, easy to use and provide fully acceptable values of monitoring like sensitivity, LOD, LOQ, etc. In order to construct an optical biosensor, AChE is immobilized as a target enzyme for organophosphorus pesticides (OPPs) onto new membranes synthesized by a sol-gel technology. The designed biosensensor is tested for determination of chlorpyrifos-methyl, parathion, pirimiphos-methyl and diazinon in liver samples. An appropriate method for sample preparation is also developed. The new method is validated in accordance with document SANTE/11945/2015 covering the required criteria for pesticides determination. The biosensor developed can detect levels lower than 30μg/ kg. The latter is the maximum residue limit (MRL) for pesticides in food. Km of 2,2x10-3M for acetylcholine and 4 months life-time of the biosensor are obtained.