EFFECT OF STORAGE STABILITY ON THE ANTIMICROBIAL ACTIVITY OF MICROENCAPSULATED VIA SPOUTED-BED ROSEMARY EXTRACTS
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2020-01-01
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Abstract
Long-term stability of microencapsulated via spouted-bed rosemary hydro-alcoholic extracts was studied regarding their antibacterial activity. The samples were stored in dark place at 4°C for 10 months. Five batches with variation in the incorporated extracts and composition of the coating agent were tested by means of the agar diffusion method against two model strains, the Gram-negative Escherichia coli K12 and the Gram-positive Bacillus subtilis 356. Inhibitory effect was obtained from all five samples against the Gram-positive bacteria. None of the studied products showed activity against the Gram-negative strain. The most prominent effect against B. subtilis 356 was detected by microcapsules of batch 5 containing the highest amount of fresh rosemary extract, followed by the samples with concentrated extract (batch 2 and 4). The least inhibitory effect was observed by the microcapsules of batch 1 and 3, containing a lower quantity of fresh non-concentrated extract